People and projects

The Lab – July, 2016

July2016                                  The lab (and Helix the cat): summer of 2016.

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Carolyn and Helix

Carolyn Brown


You can find a listing of Carolyn’s publications on Google Scholar (so much easier than searching Pubmed for C Brown!)

Ph.D., 1991, University of Toronto

Postdoctoral Fellowships:

  • Stanford University and
  • Case Western Reserve University

Current lab members

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Happy Birthday!
Samantha Peeters, B.Sc. (Guelph) is a Ph.D. student identifying the DNA elements allowing human genes to escape X-chromosome inactivation.  She is doing this by integration of human DNA into the mouse X chromosome in collaboration with the Simpson lab at CMMT.  She is currently using CRISPR in a mouse embryonic stem cell line with two X chromosomes to generate a female cell line for targetting.

Tom & X
Tom and X image in LSI
Thomas Dixon-McDougall, B.Sc. (Toronto) is a Ph.D. student studying the domains of the XIST RNA that are critical for the RNA to localize in cis, recruit chromatin modification, and silence flanking genes.  He is currently using CRISPR to generate deletions along the X chromosome and uses FISH (he generated the ‘X’ on the header); immunohistochemistry; and allelic analyses to examine the impact of the deletions.
Brad Bradley Balaton, B.Sc. (Saskatoon) is a Ph.D. student studying genes that are variable in their escape from X-chromosome inactivation, so that they are subject to inactivation in some females, but escape from inactivation in other females.
Christine Christine Yang, (M.Sc. UBC) did her M.Sc. on using DNA methylation to examine silencing of transgenes.  She then undertook the lab’s first RNA and ChIP-seq studies (see Genome Biology paper) and has become the lab’s RIP-star.

Calico Gashapon
Gashapon calico Brad brought from Japan
Sarah (B.Sc. Simon Fraser University) is the lab manager and is looking at XIST RNA:protein interactions.
OLYMPUS DIGITAL CAMERA Diana, a summer intern with the MITACs program, is studying the ‘boundaries’ between genes that are subject to silencing and those that escape X-chromosome inactivation.

Graduates and their Theses

Andrew Chapman, M.Sc., 2013
Transcriptional regulation of the XIST locus
Angela Kelsey, M.Sc., 2013,
Mechanisms for XIST RNA cis-localization
Jakub Minks, Ph.D., 2012
Role of XIST and its interacting partners in gene silencing
Allison Cotton, Ph.D., 2012
Patterns of DNA methylation on the human X chromosome and use in analyzing X-chromosome inactivation
Christine Yang, M.Sc., 2012
DNA methylation demonstrates spread of X-chromosome inactivation to human transgenes
Nancy Thorogood, Ph.D., 2010
XIST/Xist Induced Epigenetic Events in Somatic Cells
Samuel Chang, Ph.D., 2009
Identification and characterization of cis-acting regulatory elements for human XIST
Desiree Quint, M.Sc., 2006
Aberrant Hypermethylation of Xq12 in Lymphoma
Ziny Yen, M.Sc., 2005
Comparative studies of X inactivation within eutheria
Jennifer Chow, Ph.D., 2003
XIST and its role in X-chromosome inactivation
Ed Kwok, M.Sc., 2002
XIST and macroH2A1.2 in hybrid cells
Cathy Anderson, Ph.D., 2002
Variable X inactivation of the human TIMP1 gene
Sanja Karulic, M.Sc., 2001
Studies of X-chromosome inactivation and identification of the Xist gene in the insectivore Scapanus orarius