Publications

 

Recent Lab Papers Synopsis
2016BalatonTIGS

http://www.ncbi.nlm.nih.gov/pubmed/27103486
12-20% of human genes escape inactivation and continue to be expressed from the otherwise inactive X chromosome. These exceptions to silencing can inform our understanding of the silencing process, and are also differentially expressed between males and females, so may be important contributors to sexually dimorphic disease predispositions. In this review we tabulate the differences in epigenetic marks between the escape and subject genes, review how escape genes have been identified, and describe recent results about the nuclear ultrastructure of the inactive X chromosome.
1-s2.0-S1084952116300416-main_Page_1

http://www.ncbi.nlm.nih.gov/pubmed/26849907
X-chromosome inactivation is generally a random choice of one or the other X to inactivate, resulting in females being mosaics of cells with each X active (the Calico Cat is our usual example).

But inactivation is not always random – and skewing is important for modulating the disease severity of female carriers of X-linked disease. Therefore we would like to understand how skewing occurs.

Humans and mice both undergo X-chromosome inactivation; but there are important differences between the two species, and in this review we discuss whether there is a region controlling skewing of inactivation in humans, as there is in mice.

2015-Kelsey_Integrations

http://www.ncbi.nlm.nih.gov/pubmed/26429547
We have previously shown that induction of XIST in a human somatic cell can result in localization of the RNA and recruitment of some features of an inactive X.

In this study we have put the inducible XIST into 9 different places in the human genome and while they all localize XIST, the site of integration impacts how well silencing can be established.

Silencing can skip over regions, and seems to favour places that already have some pre-existing H3K27me3.  All sites recruited H2A ubiquitinylation and showed a shift to the perinuclear periphery upon XIST induction.

2015-Cotton X DNAm Landscape_Page_01

http://wwww.ncbi.nlm.nih.gov/pubmed/25381334
DNA methylation is found at the CpG island promoters of X-linked genes subject to X-chromosome inactivation.  In order to identify genes that are subject to inactivation we have surveyed datasets from 27 different tissues to compare male (who only have an active X) and female (who have an inactive X in addition to the active X) levels of DNA methylation.

The level of DNA methylation is very consistent across tissues, and we see a very strong inverse correlation with expression at the promoter, and a smaller correlation in the gene body, allowing us to ‘call’ more escape genes.  In summary:

AC's 450K paper figures

2014-Chapman P2 BMC Genetics_Page_01

http://www.ncbi.nlm.nih.gov/pubmed/25200388
Expression of the XIST gene initiates X-chromosome inactivation.  But what flips the XIST switch?  In mice there is an antisense to XIST that regulates XIST expression, but the region around XIST is very different between humans and mice.  Yet both humans and mice have a second major promoter “P2” embedded within the gene.  Surprisingly P2 would not create a functional XIST RNA as it is downstream of the critical “A repeats” that are essential for silencing.  Could P2 be a common regulator of XIST/Xist?  We show that P2 is located in a differentially methylated set of YY1 binding sites and that YY1 is critical for XIST expression and localization
2013-CottonHum Mol Genet X-A_Page_01

http://www.ncbi.nlm.nih.gov/pubmed/24158853
X-chromosome inactivation can spread into DNA that is adjacent to the XIST gene, even if that DNA is an autosome translocated to the X.  We examined the spread of inactivation into the autosomal DNA in unbalanced X/autosome translocations using DNA methylation to identify silenced genes. The spread of silencing on the autosome was less complete than on the X chromosome, consistent with the 1983 proposal by Riggs and Gartler of ‘waystations’ that are more abundant on the X than other chromosomes.  We show that spread is entriched at regions with pre-existing EZH2 or other heterochromatic marks, while escape from silencing is best correlated with the presence of ALU repeat elements.
Collaborative Studies Description
2017-chen-scientificreports

https://www.ncbi.nlm.nih.gov/pubmed/27857184
 nature-2013-jiang_page_1

http://www.ncbi.nlm.nih.gov/pubmed/23863942

The Lawrence laboratory at U. Massachusetts are expert cell biologists who were the first to visualize XIST in the nucleus.

Here, they are the first to approach the idea of ‘chromosome therapy’ – that if XIST silences in cis, then integration of XIST will ‘correct’ a trisomy by silencing the extra copy.

XIST was able to silence a chromosome 21 in Down’s syndrome iPS cells – really well, according to DNA methylation!

 srep10423_page_01

http://www.ncbi.nlm.nih.gov/pubmed/26013764

We have collaborated with the Lam laboratory on many studies looking at DNA methylation – but additional roles for RNAs in controlling aberrant gene expression in cancer cells is a new avenue that Victor pursued.
 art%3a10-1186%2f1756-8935-6-4_page_01

http://www.ncbi.nlm.nih.gov/pubmed/23452981

The Robinson lab are also long-time collaborators.  Here Magda and Allison got together to create an annotation of the 450K DNA methylation array.